C9745
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 | Immunofluorescence 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Cullin 3 (C-terminal) (Cat. No. C9745). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. |
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C9745
Sigma
Anti-Cullin 3 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-CUL3 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 751-768 of human Cullin 3, conjugated to KLH. The corresponding sequence is identical in mouse and rat. |
| Physical form | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~80 kDa |
| species reactivity | human, rat, mouse |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 2-5 μg/mL using whole extract of human Jurkat cells |
| immunoprecipitation: 1-2 μg using lysate of rat brain | |
| indirect immunofluorescence: 2-5 μg/mL using mouse 3T3 cells | |
| Gene Information | human ... CUL3(8452) |
| rat ... Cul3(301555) | |
| mouse ... Cul3(26554) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
Related Products
| Related product | C0871, Anti-Cullin 3 antibody produced in rabbit |
